Requirements for Membrane Attack Complex Formation and Anaphylatoxins Binding to Collagen-Activated Platelets
نویسندگان
چکیده
منابع مشابه
Requirements for Membrane Attack Complex Formation and Anaphylatoxins Binding to Collagen-Activated Platelets
BACKGROUND The activation of complement during platelet activation is incompletely understood. OBJECTIVES We sought to explore the formation of C5b-9 and anaphylatoxins binding to collagen-activated platelets. METHODS C5b-9, anaphylatoxins C3a, C4a and C5a, and anaphylatoxin receptors C3aR1 and C5aR were measured by flow cytometry and/or confocal microscopy. Platelet microparticles were qua...
متن کاملHeparin binding to resting and activated platelets.
Heparin inhibits platelet function and can cause thrombocytopenia. In an effort to understand these phenomena, we have measured the binding of (3H)-heparin to resting and stimulated platelets. In platelet-rich plasma, a single class of saturable heparin binding sites was observed (apparent dissociation constant [kd] approximately 0.55 microgram/mL, R approximately 0.059 microgram/10(9) cells). ...
متن کاملBinding of radiolabelled collagen to blood platelets.
Purified, soluble collagen was prepared from rat skin, and the tyrosine residues were labelled with I by the chloramine T method. The collagen was added to platelet-rich plasma from rats or rabbits, and then layered on 23 % w/v Ficoll. After centrifugation at 3900 g for 20 min, the platelets were recovered from beneath the Ficoll, and the radioactivity associated with them was measured. This re...
متن کاملFactor XIIIa Binding to Activated Platelets
Stabilization of a clot is dependent on fibrin cross-linking mediated by the transglutaminase, factor Xllla (FXllla). In addition to fibrin stabilization, FXllla acts on a number of platelet-reactive proteins, including fibronectin and vitronectin, as well as the platelet proteins, glycoprotein (GP) Ilb-Ma, myosin, and actin. However, conditions inducing the platelet-activation dependent bindin...
متن کاملStructural basis of complement membrane attack complex formation
In response to complement activation, the membrane attack complex (MAC) assembles from fluid-phase proteins to form pores in lipid bilayers. MAC directly lyses pathogens by a 'multi-hit' mechanism; however, sublytic MAC pores on host cells activate signalling pathways. Previous studies have described the structures of individual MAC components and subcomplexes; however, the molecular details of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2011
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0018812